OPTOX

Development and Validation of Bio-Optical Ecotoxicological Tests in Marine Phototrophs

Objective

The OPTOX Project aims to apply non-invasive high-throughput screening (HTS) techniques, namely pulse amplitude modulated (PAM) fluorometry, laser induced fluorescence (LIF) and spectroradiometry, as tools for ecotoxicology assessment in marine phototrophs exposed to emerging contaminants. These tests will be applied in model organisms, representative of major marine authotrophic groups (microalgae, macroalgae and marine angiosperms such as seagrasses and halophytes), and integrated through artificial intelligence (AI) for ecotoxicological indexes development. Allied to this innovative bio-optical approach, biochemical/metabolic tests will also be performed in exposed organisms, allowing confirmation of the physiological stress conditions.

Impact

This multidisciplinary approach intents to produce innovative ecotoxicology tests of great added value for future management requirements and impact assessment.

INOV Participation

Elaboration of the methodology, as well as the equipment and software, for experimental evaluation of the stress related to contamination, using the laser-induced fluorescence emission spectrum.